[DSMZ 공식대리점 관련 업무대행/수입] L-929 제품소개

어스바이오는 DSMZ 전문 취급점으로서 모든 제품을 전문적으로 취급 및 공급하고 있습니다.
DSMZ의 Cell line을 소개드립니다.
 

Cell line: L-929
DSMZ no.: ACC 2
Species: murine (mouse) (Mus musculus)
Cell type: connective tissue fibroblasts
Biosafety level: 1

DSMZ Cell Culture Data:
 

Morphology: fibroblasts growing as monolayer
Medium: 90% RPMI 1640 + 10% h.i. FBS
Subculture: seed out at about 2.0-3.0*10⁶ cells/80 cm² , split confluent cultures 1:5 to 1:10 using trypsin (do not use trypsin/EDTA), after 2-3 days monolayer will be confluent, split 2-3 times a week
Incubation: at 37 °C with 5% CO2
Doubling time: ca. 20-24 hours
Harvest: ca. 15*10⁶ cells/80 cm²
Storage: frozen with 70% medium, 20% FBS, 10% DMSO

DSMZ Scientific Data:

Mycoplasma: negative in DAPI, microbiological culture, PCR assays​​​​​​​
Species validation: Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Mus musculus species.​​​​​​​
Cytogenetics: murine hypertriploid karyotype - 61-67, 12-16 centric fusion markers present.
Viruses: ELISA: reverse transcriptase negative, PCR: SMRV-
 

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